Instant Freeze-dried OP50

Simple. Convenient. Reliable.

Don't settle for the inconsistency of in-house grown OP50. Use Freeze-dried Instant OP50 from LabTIE to obtain reproducible food quality and lawn thickness. Freeze-dried Instant OP50 is in ready-to-use format to trigger steady feeding behavior over time.
 

C. elegans Nutrition

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Note: 1 unit/vial will feed C. elegans in a 250 mL liquid culture or seed 100 plates.
 

LabTIE Freeze-dried OP50 enables you to:

  • Remove the variable of ‘quality of food/nutrition’ from the equation in your experiments
  • Feed C. elegans with a specific and measurable amount of nutrition
  • Eliminate the time and effort needed to grow your own OP50

Instant Freeze-dried OP50 is a high quality cultured Escherichia coli and is used as food source for the nematode Caenorhabditis elegans. It is used to seed NGM agar plates, add to liquid cultures (S-medium) or used to feed C. elegans in microfluidics chips.

  
  Advantages:

 

  • High and consistent quality

  • Direct use of NGM Petri dishes after inoculation

  • Freeze-dried (easy storage)

  • Compatible with microfluidic chips

  • Compatible with liquid cultures

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Video shows the preference of C. elegans for freeze-dried OP50

The video shows the preference of C. elegans for this freeze-dried OP50 as a convenient food source. Consistent quality enables reproducible results for experiments and technical replications. It is freeze-dried and has single cells when dissolved in liquid.

  Freeze-dried OP50 triggers steady feeding behavior over time
 

Figure 1. Freeze-dried Instant OP50 triggers steady feeding behavior over time[1].
Pharyngeal pumping frequency over time as elicited in Day-1 adult C. elegans. Worms were starved for 2 hours on a plate prior to 7-min incubation in re-hydrated freeze-dried bacteria (1.52mg/mL). n = 4 to 9 worms per time point.

 

Product reviews

logo riken center for sustainable resource science   logo mdi biological laboratory  
 Yasuhiro Kadota    Dustin Updike    Carole Couillault
 CSRS    MDI Biological Laboratory    IBDM
         
The freeze-dried OP50 is amazing! C. elegans grows very nicely with it. I do not need spend my precious time just to grow OP50 and collect it by centrifugation any more in the rest of my life.   LabTIE’s freeze-dried OP50 allows us to start C. elegans liquid cultures on demand. This has been a game changer, removing much of the prep time needed for growing bulk quantities of worms for gene expression and proteomic studies.   I used the Freeze-dried OP50 to do my liquid cultures to obtain huge amount of worms or embryos for specific experiments. With this food, worms are healthy and never contaminated. It's a very useful system that I recommend without hesitation.

 

 
Product photos

 

Freeze-dried OP50 is a high quality cultured Escherichia coli   Freeze-dried OP50 is a high quality cultured Escherichia coli
 
  Freeze-dried OP50 is a high quality cultured Escherichia coli

 

 

 
Universities and Companies using LabTIE Freeze-dried OP50 in their labs