C. elegans Synchronizer allows you to harvest synchronized nematodes 

 

 

 

 

 

 

 

L1 C. elegans Synchronizer

Healthy, Synchronized, Phenotype free worms

 

The L1 C. elegans Synchronizer (CES) is a manual worm Synchronizer that allows you to harvest small and large volumes of tight synchronized L1’s without the use of chemicals or starvation. It enables you to get synchronized worms that do not contain phenotypes caused by bleach or food arrest. In addition the system is designed to be successfully used without extensive training and enables C. elegans experiments with healthier worms than before, giving you the freedom to focus on the experiments by eliminating the need for worm controls and variable outcomes.

 

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Advantages:

  • Always Healthy Worms, increasing High Reproducibility of your data

  • In only 15 minutes

  • Phenotype free worms, Synchronized without Chemicals and Starvation (arrest)

  • No training required

  • Larger L1 harvests than ever before

 

 

 

 

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 Video shows how to use the L1 C elegans Synchronizer

  Nematodes contain no phenotypes caused by bleach or food arrest
This video shows how to use the L1 C elegans Synchronizer.  

Figures: Results showing an average of 99.91% purity of L1’s when using the LabTIE L1 C. elegans Synchronizer (CES).
Using the CES, the amount of L1’s present in the harvest samples compared to other stages (anomalies) was identified to determine the accuracy of the Synchronizer. A 50mL F3 generation S-medium culture was used, containing a C. elegans mixed population and was synchronized using the CES. The culture was washed for 15 minutes prior to 90 minutes of harvesting L1’s in S-media without OP50.

(A): After synchronizing, the total amount of worms were counted from 10 harvesting experiments.
(B): Anomalies were counted in each harvesting experiment. Results were plotted against the total amount of worms.
Example harvesting experiment 1: 6400 L1 worms vs 10 anomalies (7 x L2's, 3 x L3's) = 100-(100/6400*10) = 99.84% L1 purity in harvest compared to other stages present.

 

 

 

 
Product photos

 

Synchronized nematodes without the use of chemicals or starvation   Synchronized nematodes without the use of chemicals or starvation
 
  Synchronized nematodes without the use of chemicals or starvation