The ScreenChip™ System

Synchronize and Associate Visual Phenotypes with Feeding Behavior

 

To understand the mechanisms of action of your proteins or drugs, it is important to understand the behaviors (social behaviors, reward behaviors, sickness behaviors, and avoidance behaviors) of overall worm populations as well as phenotypes (specific phenotypes such as unc, dpy, dumpy, RNAi, and blister) that reveal the underlying cellular and molecular forces in action. The ScreenChip™ System, along with the WMicrotracker System, allow you to get in-depth understanding of worm behavior.

 

The ScreenChip™ System, a C. elegans visual screening and phenotyping platform allows you to associate visual phenotypes with feeding behavior.

  • Measure, visualize and analyze the neuromuscular aspects of feeding behavior
  • Assess the neuronal and physiological responses to drugs, aging, genetic modifications or environmental changes (Fig. 1 & 2)
  • Perform multiple phenotyping analyses at once, i.e., morphology, fluorescence, defecation, and feeding behavior while your worm is immobilized in the chip
  • Eliminate experimenter’s bias and get more reproducible data

 

With the ScreenChip System, you can:

  • Record videos that are perfectly synchronized to the EPG traces, all with a single software.
  • Observe and playback all the molecular and cellular events in the pharynx to better understand what is happening to your worms.

All you need is a microscope (inverted or dissection), a vacuum source and a computer that meets the minimum requirements for running the ScreenChip Software.

 

Please ensure that your microscope includes the following basic requirements:

  • A C-mount or CS-mount camera port
  • For magnifications 10X or 20X: inverted  
  • For 40X magnification: standard long working distance objective (higher magnification not suitable with the ScreenChip system)
  • Cold light source (LED)

 

What we use in our lab:

  • Leica M165 FC - Fluorescent dissecting microscope
  • Zeiss Axiovert - High magnification (40X and up) inverted microscope

We haven't tested other brands and models, however microscopes with similar specifications should work well with the ScreenChip system.

 

Fluorescence: The ScreenChips are 100% optically clear and compatible with all light wavelengths (excitation and acquisition).

The ScreenChip™ System

€ 0,00Price
    • Montmorency tart cherry (Prunus cerasus L.) acts as a calorie restriction mimetic that increases intestinal fat and lifespan in Caenorhabditis elegans. Davidvan de Klashorst, Ambervan den Elzen, Jasper Weeteling, Michael Roberts, Terun Desai, Lindsay Bottoms, Samantha Hughes. Journal of Functional Foods. Volume 68, May 2020, 103890.
       
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    • Electrophysiological measures of aging pharynx function in C. elegans reveal enhanced organ functionality in older, long-lived mutants. J. Russell, N. Burnaevskiy, B. Ma, M. Mailig, F. Faust, M. Crane, M. Kaeberlein, A. Mendenhall. The Journals of Gerontology: Series A, Nov. 2017.
       
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    • Rabies virus modifies host behaviour through a snake-toxin like region of its glycoprotein that inhibits neurotransmitter receptors in the CNS. K. Hueffer, S. Khatri, S. Rideout, M. Harris, R. Papke, C. Stokes & M. Schulte. Nature Scientific Reports 7, Article number: 12818 (2017)
       
    • Distinct unfolded protein responses mitigate or mediate effects of nonlethal deprivation of C. elegans sleep in different tissues.  J. Sanders, M. Scholz, I, Merutka and D. Biron. BMC Biology 2017 15:67.

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